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Relative affinity of mjaSSB for single- and double-stranded DNA. Reaction mixtures (20 μl) contained a fixed quantity of mjaSSB (1.75nM) and [32P]dT30 (0.05nM). Before incubation, the [32P]dT30 was mixed with single- or double-stranded M13 DNA to the indicated final concentration. Protein–DNA complexes were separated from free DNA by agarose gel electrophoresis, and the fraction of DNA bound to protein was estimated by quantification of digitized images of scanned autoradiograms.
