Figure 5.

Binding site size of mjaSSB. Reaction mixtures (2 ml) contained a fixed amount of mjaSSB (0.4 nmol). The indicated amounts of poly(dT) were added, and the fluorescence, at 348 nm was measured at 25°C with an excitation wavelength of 295 nm. The percent quenching is the change in mjaSSB fluorescence normalized to the initial fluorescence in the absence of poly(dT).