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Interaction of mjaSSB with oligonucleotides of different lengths. Reaction mixtures (20 μl) contained 1 fmol of oligonucleotide of the indicated length and 5 fmol of mjaSSB. After incubation at 25°C for 30 min, protein–DNA complexes were separated from free DNA by agarose gel electrophoresis. The fraction of DNA bound to protein was estimated by quantitation of digitized images of scanned autoradiograms.
