Table 1.
Homologues of eukaryotic DNA replication proteins
| Eukaryotic protein | Functional role | Archaeal homologue | Bacterial homologue |
|---|---|---|---|
| Orc1/Cdc18/Cdc6 | Initiation | Yes | ? |
| MCM complex | Initiation | Yes | ? |
| RPA (ssDNA binding) | Initiation/elongation | Yes | Yes |
| Primase | Initiation/elongation | Yes | ? |
| DNA polymerase (B family) | Elongation | Yes | Yes |
| RFC (clamp loader) | Elongation | Yes | Yes |
| PCNA | Elongation | Yes | Yes |
| DNA ligase | Elongation | Yes | Yes |
| FENI | Elongation | Yes | ? |
| Ribonuclease H | Elongation | Yes | Yes |
| Dna2 helicase | Elongation | Yes | ? |
| Topoisomerase I | Elongation | Yes | Yes |
Entries in the table are derived in part from ref. 28 and in part from blast searches with the archaeal and/or eukaryotic replication protein as query. See also ref. 6, 18, and 19 and the web sites http://www.tigr.org/tdb/mdb/mjdb/mjdb.html, http://www.tigr.org/tdb/mdb/afdb/afdb.html, and http://www.biosci.ohio-state.edu/∼genomes/mthermo/.