Table 1.
Copy numbers and adult expression levels of transgenic mice
| Mutant type
|
Wild type
|
||||
|---|---|---|---|---|---|
| Founder line | Copy number | hGH, ng/ml | Founder line | Copy number | hGH, ng/ml |
| 1A* | 1 | 470 | 1A* | 1 | 36 |
| 1B* | 1 | 530 | 1B* | 1 | 20 |
| 1C* | 1 | 1,060 | 2 | 2 | 14 |
| 2 | 2 | 650 | 3 | 3 | 22 |
| 3 | 3 | 1,260 | 5 | 5 | 5 |
| 8* | 8 | 2,990 | 10* | 10 | 13 |
| 10* | 10 | 3,360 | 13* | 13 | 187 |
| 13* | 13 | 4,650 | 100A | >100 | 1,400 |
| 15* | 15 | 5,560 | 100B | >100 | 30 |
| 19* | 19 | 6,490 | |||
The designations of founder mice integrated with wt HS40-ζGH or mt HS40(3′NA-II)-ζGH are listed in the first columns. Those founders for which lines have been established are indicated by ∗. The copy numbers of the transgenes are listed in the second columns. The levels of GHs in the sera of adult mice treated with phenylhydrazine are listed in the third columns. For hGH analysis, the mice with wt transgene were all assayed at the age of 5 months except founder 1B, which was 9 months old. Mice with the mt transgene were all assayed at the age of 4 months except founder 15, which was 2 months old.