Figure 6.

Inhibition of rBax and rBakΔC-induced cytochrome c release by inhibiting the PT. (A) Prevention of rBax-induced cytochrome c release by CsA. Mitochondria (1 mg/ml) were incubated with rBax (100 μg/ml) in the presence of CsA at the indicated concentrations. At 20 min, samples were centrifuged and aliquots (20 μl) of the supernatants were analyzed by Western blotting using anti-cytochrome c antibody. (B and C) Prevention of rBax- and BH3-peptide-induced cytochrome c release by depletion of Ca²⁺. Mitochondria were incubated with 100 μg/mg of rBax, mock proteins, or BH3 peptides derived from Bak or Bax (10 μM) under Ca²⁺-depleted conditions. At 20 min, samples were obtained as described in A and the amount of cytochrome c (cyt.c) released was analyzed by Western blotting.