Figure 7.

No requirement of cytochrome c release for rBax-induced Δψ loss. (A) Cytochrome c depletion from mitochondria. Cytochrome c was depleted by washing mitochondria with a hypotonic buffer as described in Materials and Methods. Intact mitochondria (intact) and cytochrome c-depleted mitochondria (cyt.c-depleted) (30 μg each) were analyzed for cytochrome c (cyt.c) by Western blotting. (B) rBax-induced Δψ loss in cytochrome c-depleted mitochondria with exogenous cytochrome c. Cytochrome c-depleted mitochondria (1 mg/ml) were incubated with rBax (50 μg/ml) or mock protein in the presence of exogenous cytochrome c (1 mM), and Δψ was measured using Rh123.