Figure 4.

Effects of FAT RNA on various trypanosome organelles. Cells were transfected with FAT RNA and processed for indirect immunofluorescence at the indicated times. Nuclei and kinetoplasts were stained with DAPI, tyrosinated α-tubulin was decorated with rat mAb YL 1/2 (14) and fluorescein isothiocyanate-conjugated anti-rat IgG secondary antibody (panels YL 1/2), and the PFR of the flagellum was immunostained with mouse mAb ROD-1 (15) and a rhodamine-conjugated anti-mouse IgG secondary antibody (ROD panels). In the DAPI images, kinetoplast DNA appears as discrete dots. The bright-stained dots in the YL 1/2 images are basal bodies. Images were obtained with a 100× PlanApo/1.4 Nikon lens.