Table 2.
Proportions of trypanosome cell types as a function of time after transfection with FAT RNA
| Cell type | 0 hr | 3.5 hr | 6.5 hr | 10 hr | 20 hr |
|---|---|---|---|---|---|
| 1K1N | 84 | 85 | 79 | 64 | 22 |
| 2K1N | 12 | 12 | 5 | 5 | — |
| 2K2N | 4 | 3 | 16 | 31 | 32 |
| 4N | — | — | — | — | 46 |
Cells were transfected with FAT RNA (10 μg per 108 cells), and aliquots were fixed and stained with DAPI at the indicated times. For each time point, 150 cells were analyzed. The percentage of each cell type is indicated. 1K1N, one kinetoplast and one nucleus; 2K1N, two kinetoplasts and one nucleus; 2K2N, two kinetoplasts and two nuclei; 4N, four nuclei.