Table 2.
Effects of oxytocin antagonist, administered through different routes, on ICP responses induced by 7-OH-DPAT
| Number of ICP responses | Latency of first ICP response(s) | |
|---|---|---|
| OT antag., i.v. | ||
| 0 | 2.7±0.4 (10) | 794±156 (9) |
| 0.25 μg kg−1 | 2.6±0.8 (10) | 838±163 (6) |
| 1 μg kg−1 | 2.9±0.4 (10) | 867±82 (9) |
| OT antag., i.c.v. | ||
| 0 | 3.6±1.0 (10) | 645±135 (9) |
| 0.001 μg | 3.5±0.7 (10) | 488±77 (9) |
| 0.01 μg | 1.9±0.5 (10) | 1146±124b,d (7) |
| 0.1 μg | 0.8±0.5a,c (10) | 1354±205b,d (3) |
| OT antag., i.t. | ||
| T13 | ||
| 0 | 3.2±0.8 (10) | 654±125 (8) |
| 0.1 μg | 2.7±0.5 (10) | 793±194 (9) |
| L6 | ||
| 0 | 2.5±0.5 (10) | 933±95 (9) |
| 0.1 μg | 2.3±0.7 (10) | 898±138 (6) |
Abbreviations: ICP, intracavernosal pressure; i.c.v., intracerebroventricular; i.t., intrathecal; i.v., intravenous; 7-OH-DPAT, 7-hydroxy-2-(di-N-propylamino) tetralin; OT, oxytocin.
The OT antagonist (OT antag.) was injected via i.v., i.c.v. or i.t. route 15 min prior to 7-OH-DPAT (10 μg, i.c.v.). ICP was recorded over 30 min following 7-OH-DPAT delivery. The number of ICP responses was counted during the 30-min 7-OH-DPAT post-injection period. The latency of the first ICP response after i.c.v. delivery of 7-OH-DPAT was also determined. The values are the means±s.e.mean of data from n number of rats. Statistical analysis was performed by Kruskal–Wallis+Dunn's post hoc test for comparison of the number of ICP responses: aP<0.05, different from corresponding control; cP<0.05, different from OT antag., 0.001 μg. One-way ANOVA+Newman–Keuls' post hoc test for comparison of latency of the first ICP response: bP<0.01, different from corresponding control; dP<0.01, different from OT antag., 0.001 μg.