Figure 1.

Treatment of ECs with increasing doses of RNase T1 or A. RNA-labeled EC³² or EC²⁰ was incubated with the indicated concentrations of RNase T1 or A, respectively, at the temperatures indicated. Cleavage was stopped with the addition of phenol (lanes 2–7 and 9–14) or SDS (lane 15). Here and in the other figures, RNA sequences are shown next to the autoradiographs; asterisks mark the positions of labeling; arrows show the cuts introduced by the RNases; and numerical indexes indicate the positions of the residues starting from the 5′ end of the transcript. Numbers and arrows show the length of the RNA.