Figure 5.

Shortening of RNase-cleaved transcripts with GreB or PP_i and analysis of the stability and catalytic activity of the resulting ECs. The scheme on the left illustrates the format of the experiment. ECs treated with RNase T1 were incubated with GreB or PP_i and tested either for their stability in TB containing 300 mM KCl followed by separation into supernatant and pellet fractions (s and p) or for their catalytic activity (t+NTP). The sequence at the bottom shows the truncated products that were obtained.