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Kinetic plots of the off-exchange data obtained for various peptides from pepsin digestion of PKA. (a) The peptide of mass 1373.7837 (ATP-binding site). (b) The peptide of mass 1341.7118 (glycine-rich loop). (c) The peptide of mass 1628.8925 (residues 133–145). (d) The peptide of mass 1708.8816 (residues 237–250). In each graph, data for the PKA-Mg2+ATP/PKI(5–24) complex (⧫), the PKA-Mg2+ATP complex (■), and the PKA alone (•) are shown.
