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Amplification of WISP-1 genomic DNA in colon cancer cell lines. (A) Amplification in cell line DNA was determined by quantitative PCR. (B) Southern blots containing genomic DNA (10 μg) digested with EcoRI (WISP-1) or XbaI (c-myc) were hybridized with a 100-bp human WISP-1 probe (amino acids 186–219) or a human c-myc probe (located at bp 1901–2000). The WISP and myc genes are detected in normal human genomic DNA after a longer film exposure.
