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Expression of PPARγ1 activates CAP gene expression. NIH 3T3 cells infected with a retrovirus carrying PPARγ1 or vector alone were cultured in medium containing 10% FBS (lanes 1 and 6), differentiation media (lanes 2 and 7), differentiating media containing 50 μM rosiglitazone (lanes 3 and 8), medium containing 10% FBS and insulin (lanes 4 and 9), or rosiglitazone (lanes 5 and 10). Total RNA was extracted and analyzed (30 μg/lane) by RNase protection assay.
