Figure 5.

Effect of troglitazone on CAP levels in Zucker rats. (A) Zucker lean rats (+/fa) and ZDF rats were treated with troglitazone for 4 or 12 weeks as indicated. Each lane contains 30 μg of protein prepared from white adipose tissues of individual animals. The proteins were separated by SDS/PAGE followed by immunoblotting with anti-CAP antibodies. A representative Western blot using anti-CAP antibodies is shown. (B) The intensities of the 80- to 90-kDa protein were measured by densitometric scanning and were normalized against the expression of Sos examined in the same sample as a control. The results represent the mean ± SD derived from all experimental animals. L1-Adi, 3T3-L1 adipocytes cell lysates, NIH-CAP, NIH 3T3 stably transfected with an expression vector containing CAP cDNA. Values statistically significant from their respective controls are indicated by an asterisk.