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. 1998 Dec 8;95(25):14763–14768. doi: 10.1073/pnas.95.25.14763

Figure 1.

Figure 1

Telomerase activity in chicken tissue. Lanes 1–4, PCR controls. Lane 1, PCR reaction with TS and CX primers but no input from the telomerase extension reaction; lanes 2 and 3, buffer rather than extract was used in the telomerase extension reaction. Lane 4, no TS primer was added to the telomerase extension reaction. Lanes 5–12, tissue samples from 1-day-old chicken. Lanes 13–18, tissue from a 9-mo-old hen. Lanes 19–24, tissues from a 24-mo-old rooster. T, testis; K, kidney; S, spleen; L, liver; U, lung; P, pancreas; Y, thymus; B, brain; O, ovary; H, heart. Lanes 25–26, extracts from the spleen of a 1-day-old chicken; −, no RNase A; +, pretreated with RNase A. Lane 27, extract from mouse liver. Lanes 28 and 29, extract from chicken DT40 cells, an avian leukosis virus-transformed B-cell line. +, Pretreated with RNase A; −, no RNase A. To remain in the linear range of the TRAP assay, each telomerase extension reaction contained 30 μg of extract, and 4 μl of the extension products were used in the PCR reactions.