Figure 4.
Pontin52 interacts directly with β-catenin and TBP. (A) A direct interaction of Pontin52 with β-catenin and TBP was demonstrated by affinity precipitation of recombinant His6-tagged β-catenin or His6-tagged TBP with the recombinant GST-Pontin52 fusion protein. The precipitate was analyzed by SDS/PAGE and Western blotting with anti-β-catenin antibodies (Left) or anti-TBP antibodies (Right). Filled arrowhead indicates His6-tagged TBP; open arrowhead indicates His6-tagged β-catenin. (B) To map the binding site(s) of Pontin52 in β-catenin several recombinant GST-β-catenin fusion proteins were used to affinity precipitate in vitro-translated Pontin52. The precipitates were analyzed by autoradiography. Arrowhead indicates Pontin52.