Figure 5.

Pontin52 is a nuclear protein. Indirect immunofluorescence tests with the Pontin52-specific anti-25 antibody were performed on COS cells (A and A′). Pontin52 was found predominantly localized in the nucleus in dot-like structures. The dotted structures throughout the cytoplasm represent unspecific staining, because they also can be seen with the nonimmune sera (B and B′) or with anti-25 preincubated with recombinant GST-Pontin52 fusion protein (C and C′). (A′, B′, and C′) Hoechst 33342 staining of the same nuclei. (Bar: 20 μm.)