Figure 6.

Pontin52 exhibits a widespread expression pattern. (A) Western blot analysis of cell lysates from human and mouse cell lines with Pontin52-specific antibody (anti-24). An equal number of cells for each cell line was taken for comparing the relative amount of Pontin52 protein. Arrowhead indicates Pontin52. (B) Northern blot analysis on human tissues probed with a 0.69-kb BglI–EcoRV fragment of the Pontin52 cDNA. A REAL blot was purchased from Invitrogen, which as indicated by the manufacturer had been loaded with 2 μg of total RNA per lane. A band expected for the size of the Pontin52 mRNA was detected in each of the tissues. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.