Figure 7.

In vivo association of Pontin52 with the LEF-1-β-catenin complex. Several combinations of plasmids were transfected into N2A cells as indicated. Pontin52-myc was precipitated from cell lysates with myc-specific antibodies, and the immunoprecipitates were analyzed by Western blotting of HA-tagged LEF-1 with anti-HA specific antibodies. The expression of transfected β-catenin and Pontin52-MYC was monitored by Western blotting of cell lysates with anti-β-catenin and anti-myc antibodies. Arrowhead indicates LEF-1-HA; ∗ indicates degradation products of LEF-1-HA as monitored in parallel experiments with anti-LEF-1 antibodies (not shown).