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. 2008 Jul 8;5(4):189–196. doi: 10.7150/ijms.5.189

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Quantitative RT-PCR confirmed the changes in gene expression identified by microarray analysis. RNA was isolated from control cell and cells grown in the presence of 1 mM iodine for 48 hours. QRT-PCR analysis of genes predicted by microarray analysis to be down-regulated (A) and up- regulated (B & C) in response to iodine treatment. Cyclophilin A was used as control for normalization. All 10 genes showed significant changes (P < 0.03) in response to iodine treatment in concordance with the array data. The mRNA level in the control samples was set to 1 and the fold change is shown. In panel C control bar is not visible. Dark bars represent control samples while grey bars represent samples treated with 1.0 mM Iodine. Standard Deviation bars are shown.