Table 1.

MCF7 cells were treated with Lugol's iodine solution or vehicle alone for 48 hr (see Methods and Materials for details). RNA was isolated and subjected to Microarray Analysis (see Methods and Materials for details). 29 genes were upregulated ≥ 2.0-fold (A) and 14 genes were down regulated ≥ 2.0-fold (B) in response to treatment. Genes were than clustered into functional categories using the DAVID Bioinformatics Database Gene Functional Classification Tool (NIAID/NIH). The fold change in expression is relative to control cells. Bold genes were verified by qRT-PCR (Figure 2).

A. 29 Genes that are Up-Regulated1 in Response to Iodine Treatment2
Cell Cycle/Proliferation			Fold Change
NM_001673	ASNS	asparagine synthetase	4
L24498	GADD45A	growth arrest and DNA-damage-inducible, alpha3	2
Steroid Metabolism
NM_000104	CYP1B1	cytochrome P450, family 1, subfamily B, polypeptide 13	11.3
NM_001353	AKR1C1	aldo-keto reductase family 1, member C13	6
NM_000499	CYP1A1	cytochrome P450, family 1, subfamily A, polypeptide 13	2.5
Transcription
NM_003900	SQSTM1	sequestosome 1	2.8
AB025432	TSC22D3	delta sleep inducing peptide, immunoreactor	2.6
DNA repair
NM_019058	DDIT4	DNA-damage-inducible transcript 4	2.2
L24498	GADD45A	growth arrest and DNA-damage-inducible, alpha3	2.0
Lipid Metabolism
NM_000693	ALDH1A3	aldehyde dehydrogenase 1 family, member A3	3.2
NM_004315	ASAH1	N-acylsphingosine amidohydrolase (acid ceramidase) 1	2.2
tRNA synthesis
NM_004184	WARS	tryptophanyl-tRNA synthetase	3
NM_002047	GARS	glycyl-tRNA synthetase	2.3
NM_003680	YARS	tyrosyl-tRNA synthetase	2
Other
NM_021158	TRIB3	tribbles homolog 3 (Drosophila)	4
NM_005218	DEFB1	defensin, beta 1	3.3
NM_033197	C20orf114	chromosome 20 open reading frame 114	2.7
NM_004753	DHRS3	dehydrogenase/reductase (SDR family) member 3	2.7
NM_002083	GPX2	glutathione peroxidase 2 (gastrointestinal) 3	2.5
NM_006636	MTHFD2	methylene tetrahydrofolate dehydrogenase	2.5
AF104032	SLC7A5	solute carrier family 7, member 5	2.4
NM_004864	GDF15	growth differentiation factor 15	2.2
NM_000416	IFNGR1	interferon gamma receptor 1	2.1
NM_002356	MARCKS	myristoylated alanine-rich protein kinase C substrate	2.0
Function Unknown
AK001064	LOC86026	hypothetical protein DKFZp434P055	3.5
AK054816	ORAOV1	oral cancer overexpressed 1	3.0
NM_006470	TRIM16	tripartite motif-containing 16	2.4
AF245505	MXRA5	matrix-remodelling associated 5	2.3
AL162069	LOC144501	hypothetical protein LOC144501	2.2
BE884686	LTB4DH	leukotriene B4 12-hydroxydehydrogenase	2.1

B. 14 Genes that are Down-Regulated4 in Response to Iodine Treatment2
Estrogen responsive genes			Fold Change
NM_003225	TFF1	trefoil factor 1 (estrogen-inducible sequence) 3	-2.3
NM_003881	WISP2	WNT1 inducible signaling pathway protein 23	-2.4
Cell Cycle genes
NM_003258	TK1	thymidine kinase 1, soluble	-3.7
NM_002305	LGALS1	lectin, galactoside-binding, soluble, 1 (galectin 1)	-2.6
NM_007019	UBE2C	ubiquitin-conjugating enzyme E2C	-2.5
NM_001071	TYMS	thymidylate synthetase	-2.3
NM_053056	CCND1	cyclin D1 (PRAD1: parathyroid adenomatosis 1) 3	-2.0
NM_002466	MYBL2	v-myb myeloblastosis viral oncogene homolog like 23	-2.2
Cell Growth/Proliferation Genes
NM_005264	GFRA1	GDNF family receptor alpha 13	-2.5
NM_007161	LST1	leukocyte specific transcript 1	-2.5
Ion Transport Genes
NM_004669	CLIC3	chloride intracellular channel 3	-2.2
Chromatin Organization
NM_002105	H2AFX	H2A histone family, member X	-2.2
Function Unknown
NM_007173	PRSS23	protease, serine, 23	-3.7
NM_006408	AGR2	anterior gradient 2 homolog (Xenopus laevis)	-2.1

¹ Increase ≥ 2.0-fold relative to control

² Accession number is to left, followed by gene symbol, name, and fold change.

³ Verified by QRT-PCR

⁴ Decrease ≥ 2.0-fold relative to control