Figure 3.

mRNA containing the UTR(3.9) is translated in vitro ≈14-fold more efficiently relative to mRNA containing the UTR(4.1). (A) The two SP6 constructs were linearized with BamHI and transcribed in vitro by using SP6 polymerase. An equal amount of each RNA (0.5 μg) was translated by using the rabbit reticulocyte lysate system as described in Materials and Methods. Aliquots (5 μl) from each reaction were assayed for LUC activity at the indicated times. A representative time course is shown. (B) The ratio between the LUC activity generated by the translation of the UTR(3.9)–LUC mRNA relative to that of the UTR(4.1)–LUC mRNA is shown at different time points. Data were averaged from three independent sets of in vitro-transcribed RNA.