Table 1.

Effect of the UTR(3.9) and UTR(4.1) on translation in vivo

Exp.	UTR(3.9)/UTR(4.1)*
	24 hr	48 hr
1	2.4	3.3
2	2.6	2.3
3	2.6	2.6
4	nd	2.8
5	nd	2.9

*SV40–UTR(3.9)–LUC and SV40–UTR(4.1)–LUC were cotransfected with the reference β-galactosidase plasmid pON260 into COS-1 cells as described in Materials and Methods. In five separate experiments, LUC activity was measured at 24 and 48 hr posttransfection and normalized to β-galactosidase activity. The numbers represent the ratio between LUC activity measured in cells transfected with the SV40–UTR(3.9)–LUC construct relative to the LUC activity measured in cells transfected with the SV40–UTR(4.1)–LUC construct. nd, not determined.