Table 1.

Aminoacyl loading and transfer rate constants.^a

Peptideb	Active site residues	k1(10−3sec−1)	k2(10−4sec−1)
1	K_HH_C	1.3	9.2
2	K_AH_C	1.0	1.3
3	K_HA_C	1.3	0.7
4	K_AA_C	3.4	0.5
5	SGly_HH_Cc	1.3	9.1
6	SAla_HH_Cc	0.9	0.4
1	K_HH_Cd	0.5	0.03
7	Aba-SKL-CO2H	~e	0.0017
8	K_HH_S	~e	0.09
9	K_AH_S	~e	0.02

^aReaction conditions are as described in Fig. 2.

^bSee Table S1 for peptide sequences.

^cAminoacyl-esterified Ser residue (Type-II).

^dIn 6 M Gnd.HCl, pH 7.0.

^eTransthiolesterification not possible.