Figure 5.

CS-17 IgG and Fab functional effects on TSHR mutations. The same HEK293 cell lines stably expressing the TSHR mutants depicted in Fig. 4 (nonhomologous human amino acid residues converted to their porcine TSHR counterparts) were incubated with the indicated concentrations of CS-17 IgG or Fab. Only the amino acid positions are indicated; substitutions are shown in Fig. 3B). UnTx, Untransfected. Bars represent the mean ± sd of values from duplicate wells. This experiment was repeated once with similar results.