Figure 8.

Effects of estrogens and aromatase inhibitors on hormone-induced maturation of croaker oocytes in an in vitro bioassay. The oocytes were primed with 10 U/ml hCG for 8–16 h and then treated with steroids for 8 h. A, Effects of E2, ICI 182,780 (ICI), and Tmx treatments (100 nm) on spontaneous (hCG-overprimed, white bars) and 20 nm 20β-S-induced (gray bars) GVBD of croaker oocytes. +, P < 0.05 compared with vehicle control (CTL, Veh) group; *, P < 0.05 compared with 20β-S alone (20β-S, Veh) group. B, Effects of different concentrations of E2 and G-1 on 20 nm 20β-S-induced GVBD of croaker oocytes. * and +, P < 0.05 compared with respective 20β-S alone (0) groups (Tukey’s). Control (CTL) was hCG-primed alone. C, Effects of hCG alone and hCG in the presence of E2, G-1, and aromatase inhibitors on spontaneous (hCG-overprimed, non-20β-S-induced) GVBD of croaker oocytes. AGT₁, 50 μg/ml; AGT₂, 500 μg/ml; ATD, 10 μg/ml. + and *, P < 0.05 compared with hCG alone. D, Effects of E2 and G-1 (100 nm) on the GVBD of croaker oocytes primed with hCG alone and hCG in the presence of ATD (10 μg/ml). *, P < 0.05 compared with respective hCG-alone treatment; +, P < 0.05 compared with hCG+ATD treated with vehicle (Veh). Each value represents the mean ± sem of three observations with 80–150 incubated oocytes in each observation. All assays were repeated three or more times with ovarian tissue from different donors, and similar results were obtained in each assay.