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The effect of PMA on CREB phosphorylation (pCREB) at Ser133 (A) and Ser121 (B). 4B cells were incubated for 30 min with increasing concentrations of PMA in the presence and absence of a threshold concentration of forskolin (FSK; 0.1 μm). PMA induced CREB phosphorylation at both sites with a similar concentration dependence. Data points are the mean and se of values obtained by Western blot in two experiments.
