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. 2008 Apr 3;149(7):3452–3460. doi: 10.1210/en.2008-0061

Figure 7.

Figure 7

RAS proteins are present in neurons from DRG sections in adult female OVX rats. A, Immunostaining for AGN shows cytoplasmic localization within small (short-tailed arrow) and medium (long-tailed arrow) neurons. Large neurons (open arrow) showed little to no AGN immunoreactivity. B, REN-ir is present in most small (short-tailed arrow) and medium (long-tailed arrow), and in some large neurons (open arrow). C, ACE-ir is present in medium-sized (long-tailed arrow) and small neurons (short-tailed arrow). ACE-ir was generally absent in large neurons but was strong in blood vessels (asterisk). Scale bar, 50 μm in A–C. D, Double staining of DRG sections for REN (green) and AGN (red) shows colocalization in small and medium-sized neurons (arrows), whereas others express REN or AGN alone. Scale bar, 50 μm in D.