Figure 5.

Effect of leptin on body weight (A and B) of 22-wk-old CON mice and DEL littermates (n = 5/group). Leptin was administered as described in the text. Two-way ANOVA of body weight from d 0 to d 19 indicated an effect of genotype (P < 0.0001). B, Body weight differences from d 0 were determined for the 4 d of leptin treatment and group differences tested by two-tailed unpaired t tests. C, Two-way ANOVA of food intake showed significant effects of both genotype (P < 0.0001) and time (P < 0.0001) with no interaction. Group differences in daily food intake were analyzed by two-tailed, unpaired t tests. Mice were killed after the last day of feeding measurements, and neuropeptide and Socs-3 transcripts from whole hypothalamus (D) were quantified. *, P < 0.05; **, P < 0.01. Values are means ± sem.