Figure 7.

Overexpression of Dlx3 inhibits GR-induced human αGSU promoter in LβT2 cells. A, Sequence overlap of the JRE and the GRE2. Sequence is shown from −124 to −97 of the human αGSU gene. The rounded box indicates the sequence of CRE2, squared box indicates the JRE sequence, and the oval indicates the full GRE2 with both 6-bp half-sites and the 3-bp spacer. B, Either pCI empty vector or Dlx3 expression vector (100 ng) was overexpressed in LβT2 cells that had been transiently transfected with 1.8-kb αGSUluc and the GR expression vector. The cells were serum starved overnight and then treated for 24 h with 100 nm Dex. Data represent the mean ± sem of at least three experiments performed in triplicate and are presented as fold induction relative to the vehicle control.