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. 2008 Mar 27;149(7):3656–3667. doi: 10.1210/en.2008-0042

Figure 8.

Figure 8

Dose-dependent induction of intestinal and kidney target genes by 1,25(OH)2D3 in wild-type and DBP-null mice. A single dose of 1,25(OH)2D3 (0–100 ng/g bw as indicated) was injected ip into 8-wk-old female DBP-null mice and their comparable controls. The intestine and kidneys were harvested at 6 h and processed to obtain total RNA as described in Materials and Methods. A, Analysis of Cyp24a1 (left panel) and TRPV5 (right panel) transcripts induced in kidney. Total RNA was subjected to qRT-PCR analysis using the primers documented in Fig. 2 and Materials and Methods, normalized to mβ-actin levels and plotted as a function of injected 1,25(OH)2D3 concentration. Each point represents the mean ± sem (n = 5). EC50 and r2 values for each condition are as follows: kidney Cyp24a1 (DBP+/+), 0.33 ng/g bw (0.95); (DBP−/−), 0.23 ng/g bw (0.96); kidney TRPV5 (DBP+/+), 0.09 ng/g bw (0.91); (DBP−/−), 0.02 ng/g bw (0.82). B, Analysis of Cyp24a1 (left panel) and TRPV6 (right panel) transcripts induced in intestine. Measurements were assessed as in A. Each point represents the mean ± sem (n = 5). EC50 and r2 values for each condition are as follows: intestinal Cyp24a1 (DBP+/+), 0.30 ng/g bw (0.77); (DBP−/−), 0.44 ng/g bw (0.88); intestinal TRPV6 (DBP+/+), 1.15 ng/g bw (0.55); (DBP−/−), 0.05 ng/g bw (0.67).