Figure 6.

Rp-cAMPS did not prevent FSK-induced stimulation. Recording from a single cell shows that Rp-cAMPS (10 μm), inhibitor of PKA, did not alter endogenous activity or prevent stimulation of neuronal activity by FSK (left panel). The number of peaks per minute was not modified by Rp alone (N = 3, n = 62), and in the presence of Rp, the number of peaks per minute were still increased by FSK (*, P < 0.05, Student’s paired t test). The lower panel shows changes in intracellular calcium in 14 cells recorded simultaneously during the experimental paradigm (SFM-RpcAMPS-FSK-SFM). Each row represents changes in intracellular calcium in a single cell, and dots mark significant calcium peaks. Lines indicate the time for drug applications as shown on the single-cell trace.