Table 6.
Nucleotide sugar and cell-wall carbohydrate metabolism genes differentially expressed in maize F2, F2bm3 and AS225 basal and ear internodes at silking stage.
| Contig | mRNA | Basal internode | Ear internode | |||||
| Nucleotide sugar and cell wall carbohydrates | F2 | F2bm3/F2 | AS225/F2 | F2 | F2bm3/F2 | AS225/F2 | ||
| Sucrose synthase (ZmSPS5a) | 3748394.2.2 | M97550 | 66356 | 1.08 | 0.86 | 36451 | 2.17 | 1.50 |
| Sucrose synthase (ZmSus1) | 131537.2.202 | L22296 | 62970 | 1.0 | 1.38 | 92642 | 0.96 | 2.10 |
| UDP-D-glucose dehydrogenase | 2750995.2.1 | AY103689 | 35321 | 2.18 | 1.56 | 17554 | 3.83 | 2.49 |
| UDP-D-glucose dehydrogenase | 2405245.2.2 | AF457980 | 34777 | 1.95 | 1.85 | 27367 | 2.54 | 2.48 |
| UDP-D-glucuronic acid decarboxylase (AtUXS2 ortholog) | 3042304.2.1 | AY106346 | 14116 | 5.62 | 2.53 | 12568 | 1.59 | 4.34 |
| UDP-D-xylose 4-epimerase (mur4 ortholog) | 3070703.2.1 | AY106136 | 24293 | 2.33 | 1.32 | 27434 | 3.68 | 1.33 |
| GDP-D-mannose 4.6-dehydratase (mur1 ortholog) | 2621786.2.1 | AF134575 | 62635 | 2.94 | 2.92 | 181413 | 1.32 | 0.59 |
| α-5-expansin | 3696569.2.1 | AF332173 | 21885 | 2.40 | 1.39 | 13826 | 3.36 | 2.22 |
| β-5 expansin | 2419405.2.2 | AF332178 | 18565 | 2.39 | 2.17 | 17248 | 2.46 | 1.85 |
| Xyloglucan endotransglycosylase (XTH) | 8010573.2.1 | U15781 | 25201 | 2.32 | 1.68 | 21669 | 3.29 | 1.75 |
| Endo-1.3–1.4-β-D-glucanase (ZmGnsN1) | 2192481.2.2 | AF072326 | 27390 | 1.69 | 1.88 | 19511 | 3.03 | 2.29 |
| Endoglucanase (endo-1.3-β-glucanase) | 3713002.2.1 | AY109289 | 44167 | 2.30 | 1.43 | 23557 | 3.66 | 2.08 |
| Endoglucanase (endo-1.3-β-glucanase) | 2493751.2.1 | AY111608 | 36875 | 2.43 | 1.62 | 16100 | 4.90 | 3.06 |
| Exoglucanase (β-D-glucan exohydrolase II) | 2437739.2.2 | AF064707 | 15307 | 2.39 | 1.60 | 15315 | 2.39 | 1.35 |
| Xyloglucan fucosyltransferase (AtFUT1 ortholog) | 3670899.2.1 | AW287792 | 23393 | 1.84 | 1.77 | 16818 | 3.21 | 1.91 |
| Xyloglucan fucosyltransferase (AtFUT2 ortholog) | 5351892.2.1 | AY112574 | 34273 | 2.02 | 1.27 | 21944 | 2.74 | 1.83 |
| Pectate lyase | 3198766.2.1 | CG140821 | 35864 | 2.18 | 2.19 | 78008 | 2.17 | 2.87 |
| Cellulose synthase (ZmCesA-12) | QBS7b05.xg.2.1 | AY372246 | 45366 | 1.88 | 1.41 | 42685 | 2.14 | 1.54 |
| Cellulose synthase (ZmCesA-9) | 2441542.2.1 | AF200533 | 14649 | 1.95 | 1.33 | 15755 | 2.22 | 1.58 |
Normalized expression values are given for the F2 line and values for each mutant are expressed as ratios of signal intensity compared to normal plants. Genes were considered to be significantly differentially expressed when expression ratio values were lower than 0.5 or higher than 2.0. Values between the two thresholds are indicated in italics.