Figure 8. GFAP labelling in tissue sections of prion-infected mice treated with ΨGFP or ΨPrPQ167R.

The plus (+) signs indicate that the mice were inoculated with the Me7 prion strain. Mice received two injections of either ΨGFP (A, B and C) or ΨPrPQ167R (D, E and F). The section presented here are representative of the global labelling in the cortex, both in the cannula side (A and D) or non-cannula side (C and F) and in the thalamus (B and E). Bars, 6 µM. The histogram represents the mean number of GFAP-labelled cells in one mouse per group, which was measured by counting GFAP-positive cells in five squares of 10,000 µm² each using Mercator software. The histogram represents the means±s.e.m. Statistical analysis was done using STATVIEW 5 software. Values were analyzed by ANOVA followed by the PLSD Fisher test (n = 5). Multiple comparison analysis among A, B, C, D, E, and F showed that they were all statistically significant (***, p<0.0001), except for B versus C, and D versus E versus F.