Skip to main content
. 1998 Dec 8;95(25):14833–14838. doi: 10.1073/pnas.95.25.14833

Figure 4.

Figure 4

MAPK activity is required for neural induction by bFGF. (A and B) Ectoderm from embryos microinjected with 4 ng of MKP-1 (WE) or ΔP-MKP-1 mRNA was isolated at stage 8 and treated with 150 ng/ml FGF at stage 10.5. At this stage, treatment with FGF does not elicit muscle actin expression. MAPK assays were performed after 1 hr (A), and gene expression was assayed by RT-PCR at stage 24 (B). (C and D) Ectoderm also was treated at stage 11 with 150 ng/ml FGF ± 5 μg/ml cycloheximide (CHX). Assays for gene expression (C) and MAPK activity (D) were performed after 1 hr. Ac juntreated animal cap ectoderm.