Fig. 2. Paw skin biopsy for nerve fibers.
Images show sections immunostained with anti-PGP 9.5 antibody in 50 µm cryosections from the hind limb. The arrow indicates intraepidermal nerve fibers; arrow heads indicate dermal nerve fibers (scale bar 30 µm). There is a marked reduction in IENFD in the diabetic rats (B) as compared with the non-diabetic control (A). Lower panel (C) indicates the measurement of IENFD both in non-diabetic and diabetic animals. **P<0.001 as compared to non-diabetic control animals.