Figure 4.

Src homology protein (SHP) inhibition restores phagocytic capacity of alveolar macrophages (AMs). (A) Murine alveolar and peritoneal macrophages were isolated from ICR mice and cultured overnight. Sodium stibogluconate was added to inhibit SHP activity. Media were removed and fresh media containing apoptotic Jurkat T cells were added. Compared with peritoneal macrophages (PMs), AMs exhibited significantly reduced phagocytosis at baseline (**P < 0.001). This defect was corrected by SHP inhibition (*P < 0.05 vs. resting control). Open bars, control; solid bars, SHP inhibitor. (B) AMs and PMs were isolated from 4-week-old motheaten mice or age-matched C57 BL/6 controls. After overnight culture, phagocytosis of apoptotic Jurkat T cells was assessed (*P < 0.05 for wild-type AMs compared with viable motheaten AMs). Open bars, wild-type; solid bars, motheaten.