Figure 1.

PGC-1α Is Dramatically Induced after Partial Hepatectomy

Total RNA was isolated and reverse transcribed from livers of control mice that were sham-operated or had undergone partial hepatectomy (A) from control and C/EBPβ^−/− mice that had undergone partial hepatectomy (B). cDNA was quantified using real-time PCR and normalized to TBP cDNA levels. The levels of PGC-1α detected in 0.5-, 1-, 2-, 16-, 24-, and 40-h posthepatectomy (PHX) and 0.5-, 1-, 2-, and 16-h sham PHX samples are expressed relative to quiescent liver (0 h). C, G6Pase and PEPCK cDNA were quantified using real-time PCR as in A and B in 0.5-, 1-, 2-, and 4-h PHX samples expressed relative to quiescent liver (0 h). Error bars indicate sem. N = 2–4. A, PGC-1α. **, P value < 0.001 0 h vs. 2 h, #, P value < 0.05 0 h vs. 16 h. B, PGC-1β. *, P value < 0.01, 0-h control vs. 0.5 h control, 0-h control vs. 1-h mutant, 0-h control vs. 2-h control, 0-h control vs. 2-h mutant, 0 h control vs. 16-h control, 0-h control vs. 16-h mutant, 0-h control vs. 40-h control, 0-h control vs. 40-h mutant; #, P value < 0.05 0-h control vs. 24-h mutant.