Figure 5.

Cpt1a and LCAD Expression Are Reduced in C/EBPβ^−/− Livers after Hepatectomy

Total RNA was isolated and reverse transcribed from livers of control mice that had undergone Sham surgery and from control and C/EBPβ^−/− mice that had undergone partial hepatectomy. cDNA was quantified using real-time PCR and normalized to TBP cDNA levels. The levels of cpt1a (A) and LCAD (B) detected in 0.5-, 1-, 2-, 16-, 24-, and 40-h posthepatectomy (PHX) and 0.5-, 1-, 2-, 4-, and 16-h Sham PHX samples are expressed relative to quiescent liver (0 h). Error bars indicate sem; n = 2–4. A: ***, P value < 0.01, 16-h control vs. 16-h mutant PHX; ###, P value < 0.05, 16-h control vs. 0-h control; *, P value < 0.05, 4-h mutant vs. 16-h mutant; **, P < 0.05 16-h control vs. 40-h control. B: ###, P value < 0.01, 16-h control vs. 0-h control; ***, P value < 0.01, 16-h control vs. 1-h mutant; **, P value < 0.05, 16-h control vs. 24-h control; ##, P value < 0.05, 24-h control vs. 24-h mutant; *, P value = 0.01, 16-h mutant vs. 24-h mutant.