Figure 2.

DAX-1 Does Not Affect Ligand-Induced GR Transrepression

A, COS-1 cells were transfected with a NF-κB-responsive reporter 3xMHC-Luc (200 ng) and pGL3-hRL (50 ng) in the presence or absence of NF-κB subunit p65 (25 ng), hGRα (250 ng), and DAX-1 (2500 ng). B, COS-1 cells were transfected with AP-1-responsive reporter pAP1-Luc (200 ng) and pGL3-hRL in the presence or absence of the AP-1 subunits c-Fos (12.5 ng) and c-Jun (12.5 ng), hGRα (250 ng), and DAX-1 (2500 ng). After transfection, the cells were treated with vehicle or 100 nm dex in low serum OPTI-MEM media for 20 h. Reporter luciferase activity was determined and normalized to the Renilla luciferase internal control. The mean ± se from at least three independent experiments is shown. *, P < 0.05; and **, P < 0.01 represent significant differences between vehicle and dex in samples transfected with the same constructs. Con, Control.