FIG. 2.

Transient overexpression of MEKK-1 augments cytokine-induced cell death in βTC-6 cells. βTC-6 cells transiently overexpressing GFP, GFP + wild-type MEKK-1, or GFP + mut MEKK-1 were either left untreated or treated with a mixture of cytokines (50 units/ml IL-1β, 1,000 units/ml IFN-γ, and 1,000 units/ml TNF-α) for 48 h corresponding to days 3–4 after the transfection and cell sorting procedure. The cells were then analyzed for cell death by fluorescence microscopy. Results from three separate observations are presented as means ± SE. *P < 0.05 and **P < 0.01, respectively, using one-way ANOVA and Student's t test.