FIG. 7.

MEKK-1 knockdown attenuates IL-1β–induced iNOS induction in βTC-6 cells. βTC-6 cells were treated with GL3 or MEKK-1 d-siRNA. Forty-eight hours after d-siRNA treatment, the cells were either left untreated or treated with 50 units/ml IL-1β overnight. The cells were then lysed, and proteins were separated by SDS gel electrophoresis and analyzed by immunoblotting with iNOS and ERK antibodies. The percentage of iNOS induction was calculated by relating iNOS bands to those of ERK-1. Data are presented as means ± SE for three individual experiments. *P < 0.05 using Student's t test.