FIG. 8.

MEKK-1 downregulation protects against cytokine-induced βTC-6 and mouse islet cell death. βTC-6 cells (A) and dispersed mouse islet cells (B) that had been transfected with GL3 or MEKK-1 d-siRNA were either left untreated or treated with a mixture of cytokines (50 units/ml IL-1β, 1,000 units/ml IFN-γ, and 1,000 units/ml TNF-α) for 48 h and analyzed for cell death by vital staining with bisbenzimide and propidium iodide followed by fluorescence microscopy. Results from three separate observations are shown in A and are given as means ± SE. *P < 0.05 using Student's paired t test. B: Results from eight separate observations are shown as means ± SE. *P < 0.05 using repeated measurement two-way ANOVA followed by Student's t test.