FIG. 1.
The ACP T-cell repertoire in NOD mice is I-Ag7 dependent. Both I-Ag7–containing strains, NOD and B6.g7, had peptide-reactive T-cells (A and C), compared with the I-Ak+ strains, NOD.h4 and B10.BR, that did not (B and D). The frequency of PI-1(47–64)–reactive cells detected under these conditions was ∼1/30,000 in the NOD mice and 1/50,000 in the B6.g7 mice. However, PI-1(47–64)–specific T-cells were not detected in the NOD.h4 and B10.BR mice. The T-cell response to PI-1(47–64) in NOD and B6.g7 mice was MHC class II restricted as evidenced by inhibition with the MHC class II–blocking antibody, AG.2.42.7. Data presented are representative of three independent experiments.