TABLE 1.

Human and murine proinsulin sequences

	1———————9—————————————23———-30
Human (b)	FVNQHLCGSHLVEALYLVCGERGFFYTPKT
Mouse I	FVKQHLCGPHLVEALYLVCGERGFFYTPKS
Mouse II	FVNQHLCGSHLVEALYLVCGERGFFYTPMS
	31————————————-47——49———————————61
Human (C)	RREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKR
Mouse I	RREVEDPQVEQLELGGSP GDLQTLALEVARQKR
Mouse II	RREVEDPQVAQLELGGGPGAGDLQTLALEVAQQKR
	64————————————————-86
Human (a)	GIVEQCCTSICSLYQLENYCN
Mouse I	GIVDQCCTSICSLYQLENYCN
Mouse II	GIVDQCCTSICSLYQLENYCN

The sequences of the human, mouse-1, and mouse-2 proinsulin molecules are shown. The amino acid residues in bold are cleaved off during the physiological processing of the prohormone, and C-peptide is secreted in equimolar amount with the mature insulin heterodimer. The amino acid residue numbering refers to the mouse proinsulin-1 sequence, and the PI-1(47–64) segment is underlined.