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. Author manuscript; available in PMC: 2009 Jun 13.
Published in final edited form as: Cell. 2008 Jun 13;133(6):1043–1054. doi: 10.1016/j.cell.2008.04.040

Figure 6. Rewiring histidine kinase specificity in vivo.

Figure 6

(A) Schematic of the reporter construct used to assess CpxR phosphorylation in vivo. The gfp gene is driven by a cpxP promoter, which depends on CpxR~P for activation. (B) GFP fluorescence normalized by OD600 for the cpxP transcriptional reporter strain AFS161 (envZ− cpxA− PcpxP-gfp) containing the indicated plasmids. (C) Schematic of the reporter construct used to assess PhoP phosphorylation in vivo. The yfp gene is driven by a mgrB promoter, which depends on PhoP~P for activation. The cfp gene is driven by a constitutively active promoter and is used for normalization. (D) YFP/CFP fluorescence ratio of the mgrB transcriptional reporter strain AFS237 (envZ− phoQ− PmgrB-yfp PtetA-cfp) containing the indicated plasmids. In each case, the average of measurements from three independent cultures is shown. Error bars indicate standard deviations. The pEnvZ and p(MI+loop) plasmids express full-length versions of EnvZ and the chimeras (see Figure 5), respectively.