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. 2008 Jun 30;105(27):9244–9249. doi: 10.1073/pnas.0800163105

Fig. 4.

Fig. 4.

Hsp90 inhibitors cause TβR degradation. (A) 17AAG-mediated reduction of TβR levels is restored by MG132. HEK293T cells cotransfected with FLAG-TβR and HA-Smad4 were treated with 2 μM 17AAG for 6 h, in parallel with MG132 as indicated, with TGFβ added for the last 1 h. Lysates were analyzed by Western blotting with anti-FLAG, anti-HA, anti-Smad2, anti-phospho-Smad2, and anti-actin antibodies. M, molecular weight marker lane. (B) 17AAG-mediated reduction of endogenous TβR levels is restored by MG132. DR27-TR47 cells were treated as in A and harvested for Western blotting with anti-TβRI, anti-FLAG, anti-Smad2, anti-phospho-Smad2, and anti-actin antibodies. (C) 17AAG treatment attenuates TGFβ-induced MAPK phosphorylation. HaCaT cells were treated with 1 μM 17AAG for 6 h, with TGFβ added for the last 1 h. Cells were harvested for Western blotting with anti-Smad2, anti-phospho-Smad2, anti-p38, anti-phospho-p38, anti-ERK, anti-phospho-ERK, and anti-actin antibodies.