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Secondary structure of the nucleus-encoded tRNAGln from rat. Arrowheads indicate the single-nucleotide differences between the six different isoacceptors found in the genome. Arrows indicate the position of the two oligonucleotide primers used for RT-PCR amplification (432F and 433R). These two primers can only discriminate four of the possible six isoacceptors. The same primers were used for the analysis of nucleus-encoded tRNAGln from humans.
